13 years from vague idea to pre-print!
Back in 2007, when we first had started to understand that fructose-1,6-biphosphate (FBP) is a glycolytic flux signaling metabolite, I had heard a talk from Christina Smolke suggesting that one can build RNA-based sensors for metabolites, and I thought that it would be cool to have such a sensor for FBP because we then could visualize glycolytic flux in single cells. A few years later I finally had the funding, and +5 years later, we have the sensor. This was a cool collaboration between different labs and a truly heroic effort from Alvaro and Vakil! Now, the final point is to get this published. I hope this will not take another 5 years … If you don’t want to wait (so long), here it is: https://www.biorxiv.org/content/10.1101/2020.10.11.335109v1
New lab members!
Aleksandr Pogodaev (PhD from Radboud University Nijmegen) and Andre Zylstra (PhD from Cambridge) joined the lab. Welcome Aleksandr and Andre!
And here is another pre-print!
Silke has assembled a protocol paper on how to generate huge amounts of E. coli persister cells with nutrient shifts. This paper also describes a method of how one can determine the fraction of tolerant cells with flow cytometry.
A new pre-print of the lab is out
Over the last 4 years, Joana Saldida, a PhD student of the lab, has developed a new method with which intracellular metabolic fluxes can be determined with a bigger scope and in a less unbiased manner. To accomplish this, she has combined our earlier thermodynamic-stoichiometric model with 13C flux analysis. Joana found that indeed some intracellular metabolic fluxes might be different than what we had earlier thought. The pre-print is available at Biorxiv.
Matthias obtained VICI grant
Matthias obtained a VICI grant (1.5 million Euros) from the Dutch Research Council (NWO). This is a great recognition of the excellent work done by the superstars in our lab. Let’s again break new grounds!