13 years from vague idea to pre-print!

Back in 2007, when we first had started to understand that fructose-1,6-biphosphate (FBP) is a glycolytic flux signaling metabolite, I had heard a talk from Christina Smolke suggesting that one can build RNA-based sensors for metabolites, and I thought that it would be cool to have such a sensor for FBP because we then could visualize glycolytic flux in single cells. A few years later I finally had the funding, and +5 years later, we have the sensor. This was a cool collaboration between different labs and a truly heroic effort from Alvaro and Vakil! Now, the final point is to get this published. I hope this will not take another 5 years … If you don’t want to wait (so long), here it is: https://www.biorxiv.org/content/10.1101/2020.10.11.335109v1


New lab members!

Aleksandr Pogodaev (PhD from Radboud University Nijmegen) and Andre Zylstra (PhD from Cambridge) joined the lab. Welcome Aleksandr and Andre!


And here is another pre-print!

Silke has assembled a protocol paper on how to generate huge amounts of E. coli persister cells with nutrient shifts. This paper also describes a method of how one can determine the fraction of tolerant cells with flow cytometry.


A new pre-print of the lab is out

Over the last 4 years, Joana Saldida, a PhD student of the lab, has developed a new method with which intracellular metabolic fluxes can be determined with a bigger scope and in a less unbiased manner. To accomplish this, she has combined our earlier thermodynamic-stoichiometric model with 13C flux analysis. Joana found that indeed some intracellular metabolic fluxes might be different than what we had earlier thought. The pre-print is available at Biorxiv.


Vakil is now Dr. Takhaveev

Vakil has excellently defended is his thesis and is now Dr. Takhaveev. Very well done, Vakil! Watch the defense and the speech!


Matthias obtained VICI grant

Matthias obtained a VICI grant (1.5 million Euros) from the Dutch Research Council (NWO). This is a great recognition of the excellent work done by the superstars in our lab. Let’s again break new grounds!