And here is another pre-print!
Silke has assembled a protocol paper on how to generate huge amounts of E. coli persister cells with nutrient shifts. This paper also describes a method of how one can determine the fraction of tolerant cells with flow cytometry.
A new pre-print of the lab is out
Over the last 4 years, Joana Saldida, a PhD student of the lab, has developed a new method with which intracellular metabolic fluxes can be determined with a bigger scope and in a less unbiased manner. To accomplish this, she has combined our earlier thermodynamic-stoichiometric model with 13C flux analysis. Joana found that indeed some intracellular metabolic fluxes might be different than what we had earlier thought. The pre-print is available at Biorxiv.
Matthias obtained VICI grant
Matthias obtained a VICI grant (1.5 million Euros) from the Dutch Research Council (NWO). This is a great recognition of the excellent work done by the superstars in our lab. Let’s again break new grounds!